eMSTAT Solution - Features

Polystyrene (heated and unheated) polymers were separated into two groups (heated or unheated) by multivariate analysis (PLS-DA) of the MALDI mass spectrum (Score Plot). A Loading Plot can be used to confirm which peaks (marker peaks) affect the differences between the two groups.

Using marker peaks identified by multivariate analysis to create a discriminant model and discriminate (SVM) between heated and unheated polymers in a polystyrene mass spectrum, obtained separately, resulted in correct discrimination of all polymers. By using eMSTAT Solution in combination with MALDI mass spectrometry, which can easily measure samples with large molecular weight, in addition to synthetic polymers, a wide variety of samples, such as protein, fat, or sugar samples, can be easily differentiated.

Experimental Conditions
Instrument: MALDI-8020
Sample: Polystyrene 1,000
(heated for 2 hours at 200°C or unheated)
Matrix: Dithranol
Cationizing Agent: AgTFA
Sample Preparation: The polystyrene (heated and unheated) dissolved in THF, matrix solution, and cationizing agent were spotted onto a sample plate.

Extracts from commercial beef (Tasmanian and A5/A4 grade Wagyu beef) were analyzed in a DPiMS-2020 mass spectrometer. The resulting spectra were then analyzed by PLS discriminant analysis. A Score Plot confirms grouping into three groups and a Loading Plot confirms which metabolite peaks affect grouping.

With eMSTAT Solution, spectra obtained by convenient metabolite analysis in a DPiMS-2020 spectrometer can be used to easily differentiate between differences in food, plant, and other samples, and screen for information about metabolites that contribute to differentiation.

Experimental Conditions
Instrument: DPiMS-2020
Sample: Edible beef
Ionization Solution: 50% aqueous ethanol solution
Sample Preparation: 10 μL of the suspension from homogenizing the beef for human consumption in the 50% aqueous ethanol solution was dripped onto a sample plate.