Benchtop MALDI-TOF Imaging Starter Kit - Features
All-inclusive design
The Shimadzu Benchtop imaging kit contains all you need for imaging analysis, saving you effort.
| 1) | FlexiVision™-mini ITO slides (2 packs of 25 slides) |
Single-use glass slides custom-sized to fit in MALDI-8020™/ MALDI-8030™ mass spectrometers when assembled with Adaption™-mini glass slide adapter |
|---|---|---|
| 2) | Adaption-mini carrier | Used during optical scanning to securely hold either the Adaption-mini adapter/ FlexiVision-mini ITO sample slide or the FlexiMass™-SR1 Stainless steel sample plate. Ensures the sample slide surface does not contact the scanner surface |
| 3) | FlexiMass-SR1 reusable metal sample plates (4 plates) | Reusable* FlexiMass format, engraved with the accessible sample area to aid mounting of samples |
| 4) | Slide mask | Fits over the FlexiVision-mini ITO slides during matrix coating or tissue mounting to clearly identify the usable target area |
| 5) | Adaption-mini ITO glass slide adapter | Custom glass slide holder for use with the FlexiVision-mini ITO slides |
| MALDI Solutions™ imaging acquisition licence | Provides the imaging acquisition wizard to co-register the optical image and define the area to be acquired | |
| IonView™ MALDI imaging software licence | Software for processing MALDI images. Reads data directly from MALDI Solutions |
*follow appropriate washing procedure.
Kit usage example
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FlexiVision-mini ITO slide used with Adaption-mini adapter during analysis -
FlexiVision-mini ITO slide used with Slide Mask during matrix coating -
FlexiVision-mini ITO slide (within Adaption-mini adapter) used with Carrier during optical scanning
Easy viewing of imaging results
The MALDI Solutions IonViewTM software (included in kit) provides an easy workflow for routine processing of MALDI images such as snapshots, overlays and displaying regions of interest and pixel spectra.
Analysis of endogenous compounds in fingermark with 30 µm spacing
Overlay of m/z 381 and m/z 384
Fingermark MALDI image (inset) from MALDI-8020 using 30 µm spacing on FlexiMass-SR1 reusable metal slide. The background image is the optical scan.
| Sample | Compounds in fingermark |
|---|---|
| Matrix | DHB, sublimated with Shimadzu iMLayer device |
| Measurement region | 25,600 pixels |
| Measurement time | around 3.5 hours |
| Experiment details | Laser repetition rate of 200 Hz and 100 shots per pixel |
Simple Workflow
The workflow is simple making it amenable to new users learning the technique as well as experienced imaging users.
There are three main steps in the MALDI imaging workflow after collection of tissue sections:
- Sample pre-treatment and MALDI matrix coating
- MALDI data acquisition
- MALDI data processing
Solutions are provided in each step to make an otherwise typically difficult imaging workflow into a simple user-friendly application with a successful outcome for your laboratory.
1. Matrix coating
- Automated MALDI matrix coating devices available
- Easy to use
- Simple selection of pre-set methods
- High reproducibility
- Refined crystal size improves mass and spatial resolution and ionisation efficiency
2. Data acqisition
- Easy to use imaging acquisition wizard
- FastMS offers speed
- AeonDetector offers robustness
- TrueClean means easy to clean ion source
3. Data processing
*Optional item
- IonView imaging viewing software is easy to use
- Reads data directly from instrument
- Quick to reload generated IonView files
- Separate workstation available for user convenience
- Export to imzML (for statistical analysis in IMAGEREVEAL™ MS* imaging software)
* Not included in Benchtop MALDI-TOF Imaging Starter kit
Class-leading performance
The Shimadzu imaging benchtop MALDI-TOF solution has design features well-suited to a demanding application such as MALDI imaging:
The AeonDetector has up to 7x longer life and reduced aging rate.
- Class-leading sensitivity and mass resolution of the MALDI-8020 and MALDI-8030.
- FastMS feature: the combination of the 200 Hz laser, fast sample introduction (< 3mins) and quick sample stage provides a quick analytical turnaround time (speed of up to 15 pixels per second, based on lipid imaging acquisition with mass range m/z 100-1200, 50 µm spacing and an accumulation rate of 10 shots/profile)
- Robustness provided by the class-leading long lasting laser lifetime of 2 billion shots, the self-cleaning ion optics using the patented TrueCleanTM one-click laser technology, and the longer-life AeonDetectorTM .