Calibration Curves
|
|
| ■Peaks |
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13. |
Blue dextran 2000
Thyroglobulin
y-Globulin
Transferrin
Human serum albumin
Ovalbumin
β-Lactoglobulin
Chymotrypsin
Myoglobin
Lysozyme
Ribonuclease A
Cytochrome C
Gly-Tyr |
| ■Conditions |
|
|
| Column |
： |
Shim-pack Diol Series (500 mmL × 7.9 mmI.D., 5 μm) |
| Mobile Phase |
： |
A) 10mM phosphate buffer solution (pH 7)
B) 0.2M sodium sulfate |
| Flow Rate |
： |
1.0 mL/min |
| Col. Temp. |
： |
Ambient |
| Detection |
： |
UV 280 nm |
|
Analysis of Protein Standard
|
|
| ■Peaks |
1.
2.
3.
4.
5. |
Glutamate dehydrogenase
Lactate dehydrogenase
Enolase
Adenylate kinase
Cytochrome C |
| ■Conditions |
|
|
| Column |
： |
Shim-pack Diol-300 (500 mmL × 7.9 mmI.D., 5 μm)
(P/N: 228-14776-92) |
| Mobile Phase |
： |
A) 10mM phosphate buffer solution (pH 7)
B) 0.1M sodium chloride |
| Flow Rate |
： |
1.0 mL/min |
| Col. Temp. |
： |
Ambient |
| Detection |
： |
UV 280 nm |
|
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