Shim-pack GIS Series - Features
HPLC Column Solutions
Column Chemistries
| Shim-pack GIS C18 |
Shim-pack GIS C18-P |
Shim-pack GIS RP-Shield |
Shim-pack GIS CN |
Shim-pack GIS HILIC |
|
| Bonded Phase | Octadecyl Groups | Octadecyl Groups | Octadecyl Groups | Cyanopropryl Group | Diol Group |
| Analysis Mode | Reversed Phase | Reversed Phase | Reversed Phase | Reversed Phase, Normal Phase | HILIC |
| Features | High retentivity, lower columnback pressure, high inertness | High Steric Selectivity | Embedded with a Polar Functional Group | Suitable in either reversedphase or normal phase mode | Ideal for the Separation of Highly Polar Basic Compounds |
| Particle Size (μm) | 2, 3, 4, 5, 10 | 3, 5 | 5 | 3, 5 | 3, 5 |
| Pore Size (nm) | 10 | 10 | 10 | 10 | 10 |
|
Surface Area (m2/g)
|
450 | 450 | 450 | 450 | 450 |
| Carbon Loading (%) | 15 | 29 | 9 | 14 | 20 |
| End Cap | ○ | - | - | - | - |
| pH Range | 2-7.5 | 2-7.5 | 2-7.5 | 2-7.5 | 2-7.5 |
| USP Code | L1 | L1 | L1 | L10 | L20 |
High retentivity, lower column back pressure, high inertness
Analysis Examples
Food Dyes
■Peaks
| 1. Tartrazine | 7.6 mg/L |
| 2. Amaranth | 3.8 mg/L |
| 3. Ingigocarmine | 7.6 mg/L |
| 4. New coccine | 3.8 mg/L |
| 5. Sunset Yellow FCF | 5.3 mg/L |
| 6. Naphthol Yellow S | 7.6 mg/L |
| 7. Uranine | 3.8 mg/L |
| 8. Allura Red AC | 5.3 mg/L |
| 9. Ponceau R | 7.6 mg/L |
| 10. Ponceau SX | 5.3 mg/L |
| 11. Orange I | 5.3 mg/L |
| 12. Fast green FCF | 3.0 mg/L |
| 13. Brilliant blue FCF | 3.0 mg/L |
| 14. Ponceau 3R | 7.6 mg/L |
| 15. Erythrosine | 5.3 mg/L |
| 16. Azure Blue VX | 3.0 mg/L |
| 17. Orange II | 7.6 mg/L |
| 18. Acid red | 3.0 mg/L |
■Conditions
| Column | Shim-pack GIS C18 (150 mmL. x 4.6 mmI.D., 4 μm) |
| Eluent | A) 10mM Na2HPO4 (pH6.9 ; 10mM NaH2PO4) B) CH3CN A/B = 90/10 - 50 min - 65/35 |
| Flow Rate | 1.0 mL/min |
| Detection | UV 270 nm |
| Col. Temp. | 40 °C |
| Injection Vol. | 10 μL |
Free Amino acids and Organic acids
■Conditions
| Column | Shim-pack GIS CN (250 mmL. x 4.6 mmI.D., 5 μm) |
| Eluent | 20 mM KH2PO4 (pH 4.0; H3PO4) |
| Flow Rate | 1.0 mL/min |
| Col. Temp. | 40 °C |
| Detection | UV 210 nm |
| Injection Vol. | 5 μL |