Shim-pack Velox C18
HPLC Column

C18
Shim-pack Velox C18 is a traditional end-capped C18-bonded phase which offers the highest hydrophobic retention of any Shim-pack Velox phases and is applicable to a wide range of applications such as pharmaceutical, food, environmental and clinical and neutrals at moderately low and mid-range pH.
- General purpose column for reversed-phase chromatography
 - Highest hydrophobic retention among Shim-pack Velox series
 - Compatible with moderately acidic to neutral mobile phases (pH 2-8)
 
Method Transfer for Cyanocobalamin Analysis within the USP Allowable Adjustment
The assay of cyanocobalamin (a synthetic form of vitamin B12) with 5 μm fully-porous ODS column described in the USP monograph is transferred to a new method with Shim-pack Velox C18 2.7 μm column, within USP allowable adjustments. Analytical time and solvent consumption can be saved with transferred methods while meeting the requirements of system suitability.

| Column | L/dp | Flow rate (mL/min)  | 
			N | System suitability test result (Requirement: %RSD < 2.0 %)  | 
		
|---|---|---|---|---|
| VP-ODS (5 μm, 4.6 x 150 mm)  | 
			30,000 | 0.50 | 5,244 | tR: 0.025 % Area: 0.175 % (n=6)  | 
		
| 
			 Velox C18 
			(2.7 μm, 4.6 x 100 mm)  | 
			
			 37,037 
			(+23 %)  | 
			0.50 | 9,497 (+81 %)  | 
			tR: 0.035 % Area: 0.103 % (n=6)  | 
		
| 0.93 | 4,466 (-15 %)  | 
			tR: 0.084 % Area: 0.220 % (n=6)  |