Nexera MP

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Ultra-high-speed multi-sample processing using SIL-30ACMP Multiplate Autosampler

To speed up R&D in such areas as pharmacokinetics (drug manufacturing), it has become increasingly necessary to analyze large numbers of samples at high speed and high precision. In response to these demands, the new Nexera MP Ultra High Performance Liquid Chromatograph utilizing the SIL-30ACMP Multiplate Autosampler was developed. The SIL-30ACMP achieves an ultrafast injection performance exceeding that of current models while maintaining ultralow carryover performance. In addition, up to 6 microtiter plates can be loaded, enabling a maximum of 2304 samples to be analyzed continuously. By incorporating these various functions, the Nexera MP is ideally suited as a front-end LC for LC/MS and LC/MS/MS systems.

QUICKLINKS TO FEATURES

More Speed

High-speed injection of 7 seconds enables ultrafast LC/MS/MS analysis

Achieve ultrafast LC/MS/MS analysis by combining the SIL-30ACMP, featuring the fastest injection operating time of just 7 seconds and an analysis cycle of 14 seconds, with the ultrafast positive/negative ionization switching (15 msec) and high-speed scanning (15,000 u/sec) of the Shimadzu LCMS-8030 Triple Quadrupole Mass Spectrometer.

Mobile phase: Acetonitrile aqueous solution containing 0.1 % formic acid
Flow rate: 1.2 mL/min
Column: Shim-pack XR-ODS II (30 mmL. x 1.5 mmI.D., 2.2μm)
Ionization mode: ESI(+) .

 

Improved overall analysis throughput

Even on conventional models, use of the SIL-30ACMP speeds up high-throughput analysis. The following example shows that the analysis time can be shortened by at least 2 hours when an injection is performed 1000 times using the SIL-30ACMP.

 
 

More Reliable

Ultralow (near-zero) carryover achieved without the need for rinsing

When rinsing is performed to keep carryover low, the total analysis
time sometimes increases as the number of analyses increases.
Nexera autosamplers excel in suppressing carryover even without rinsing.
 

 

Ultralow carryover even on a high-sensitivity LC/MS/MS

Ultralow carryover performance is required with
LC/MS systems. The SIL-30ACMP demonstrates
exceptional carryover performance even on
compounds such as chlorhexidine that are very
prone to adsorption. Moreover, the SIL-30ACMP
features an improved rinsing mechanism to achieve
even lower carryover.
 
 
 

Improvements in high-sensitivity analysis and quantitative precision achieved by ultralow carryover

In the batch analysis of multiple components, components with greatly differing polarities are frequently analyzed together. In cases such as this, sufficient rinsing may not be achieved using one type of rinse solution. To combat this, the SIL-30ACMP has not only been designed with a hardware structure to which components are less likely to adsorb, but also has a modified rinse mechanism designed to achieve even lower carryover.

 

 

Needle port rinsing mechanism

The needle port is one of the places on an autosampler where carryover is likely to occur. On the SIL-30ACMP, the needle port is rinsed automatically.
 

Multi-rinse mechanism supporting up to 4 solutions

The needle surface can be rinsed by a maximum of two rinse solutions and the needle inner surface can be rinsed by a maximum of three rinse solutions. Of course, rinsing with just one solution in the usual way is also possible. The rinsing order can also be set as desired.
 

Outstanding injection mechanism assures precision when injecting trace amounts

The SIL-30ACMP supports an injection volume range of 0.1 µL to 50 µL. Linearity is achieved within a broad injection range from injection of trace amounts required on a UHPLC up to the order of several tens of µL used on conventional models. The SIL-30ACMP provides excellent repeatability even in the injection of trace amounts of 1 µL or less.
 
 
As shown in the figure below, column sizes with an I.D. of approximately 2 mm, which are often used on a UHPLC, are susceptible to the influence of sample solvents, and it may be difficult to increase the injection volume. However, since the SIL-30ACMP is capable of high-precision injection of trace amounts, the influence of sample solvents can be suppressed even if pretreated organic solvent-rich samples are injected directly.
 
 
In the case of sample solvents having an elution strength greater than that of the mobile phase, the peak shape is sometimes deformed when the injection volume is increased.

 

More Capacity

Mixture of 3 Plates Possible

The SIL-30ACMP accommodates 6 microtiter plates (96/384MTP, DWP) or plates for 1.5 mL vials. With 96-well plates, up to 576 samples can be loaded, and with 384-well plates, up to 2304 samples can be loaded at a time. Even with 1.5 mL vials, 324 samples can be loaded, which means that large amounts of samples can be handled with sufficient margin even as a standalone unit.

 
 
Three Types of Plates Can Be Loaded

 

Designed with emphasis on open access

In an open access environment, in which two or more researchers share one system, the ability to load different plates on each rack becomes all the more advantageous. The SIL-30ACMP provides a flexible analysis environment where, for example, one person can be performing on-time analysis with 1.5 mL vials while another is loading a microtiter plate for large-volume analysis. Vials or plates can be replaced even while analysis is in progress. Moreover, the SIL-30ACMP is designed to prevent your hands from coming into contact with the arm or other moving parts (closed-type design) while achieving open access. This prevents unexpected accidents during sample replacement from happening.

 

Analysis efficiency enhanced by building an open access environment

Combining the SIL-30ACMP, which allows samples
to be loaded at any time except when samples are
being injected, with Open Solution open access
software, creates an environment in which two or
more users can share one system. Having one
system saves energy and reduces running costs.
Because Open Solution uses Microsoft Corporation's
Internet Explorer, there is no need to install any other
special software on each PC. Moreover, there are only
three operations from login to start of analysis. Analytical
results can also be quickly checked on Internet Explorer
and easily output in predetermined formats.
 
 
 
 
 
 

Reliable performance for supporting high-precision LC/MS/MS analysis

Example of Drug Interaction Testing

With pharmacokinetics, large numbers of samples must be analyzed at high speed and with high precision. By combining the SIL-30ACMP and LCMS-8030, the advantages of the high-precision injection mechanism of the SIL-30ACMP and the reliable quantitative performance of the LCMS-8030 can be utilized, even in drug interaction tests like the one shown below.


Linearity of Nifedipine Oxide Standard Solution
 
 

 

 

 

CTO-30AS Column Oven – Minimizes the column-mass spectrometer distance –

On an LC/MS system, shortening the piping from the column to the MS is important. The optional CTO-30AS Column Oven is installed on the side face of the SIL-30ACMP. Its installation height can be adjusted (3 levels) and its angle can be adjusted (between vertical and almost horizontal) to align with the height of the detector. Since the column outlet can be aligned with the detector’s entrance and the height of the LC/MS interface, piping after exiting the column is much shorter. This minimizes peak dispersion and achieves ultrafast, high-separation analysis by making full use of the column's performance.

 

 

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For Research Use Only. Not for use in diagnostic procedures.

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