Mobile phase cleaner for HPLC

Ghost Trap DS
Mobile phase cleaner for HPLC


Suppression of ghost peaks in gradient analysis

The causes of ghost peaks are many, but often result from
impurities in mobile phases.
The Ghost Trap DS*, co-developed with Daiichi Sankyo Co., Ltd., realizes
shorter analysis time during method development and impurities
analysis by adsorbing impurities effectively in the mobile phase.
The Ghost Trap DS is applicable for the removal of impurities in
organic solvents, and is efficient in the gradient analysis of the
reversed phase condition

* DS: Abbreviation of Daiichi Sankyo (D) and Shimadzu (S)


Consistently traps impurities, even in organic solvents

A major feature of the Ghost Trap DS is the ability to remove
impurities even in organic solvents. When the Ghost Trap
DS is installed between the gradient mixer and autosampler
in reversed phase gradient analysis, it can trap impurities from
the piping and gradient mixer in addition to those from the
mobile phase. The example below shows that it can effectively
trap impurities in mobile phase, when the Ghost Trap DS is
installed just downstream of the gradient mixer as displayed in
the figure on the right.

Assess the Status of Lab Instruments in Real Time


Instrument maintenance management is required in regulated pharmaceutical laboratories. Using an iPad, maintenance data such as the status of consumables in networked instruments can be assessed at a glance instead of monitored on an instrument-to instrument basis. If parts in use exceed their control lifetimes, they are flagged with an exclamation "!" mark. Even more detailed information can be confirmed by logging in to the corresponding instrument.

Perform Column and Flow Line Cleaning Without the Workstation PC


HPLC system and analysis column cleaning are important for acquiring high-quality data. The Shimadzu HPLC web server functionality not only monitors the system, but also provides a control environment for these tasks. Using an iPad, HPLC system and column cleaning, as well as conditioning can be performed, even from a separate location.
Column  ODS column
Mobile phase A: 25 mM Phosphate (Potassium) buffer solution pH 4.0 /Acetonitrile =9/1
B: Water/Acetonitrile = 1/9
Flow rate  0.65 mL/min
Oven  45 ºC
Detection  UV 210 nm
Example of removing ghost peaks by Ghost Trap DS (The data is provided by Daiichi Sankyo Co., Ltd.)

No performance degradation even after performing analysis 500 times (equivalent to 250 hours)*1


The example below shows a baseline after gradient analysis is repeated 500 times. When compared against a case where the Ghost Trap DS is not used, it becomes clear that ghost peaks are removed by using it. Also, no ghost peak is seen in either the first or 500th analysis. The test result indicates that the Ghost Trap DS can hold trapped impurities and has enough holding capacity to be durable for long-term analyses.
Analytical conditions
System High-pressure gradient system
Column ODS column
Mobile phase A: Acetonitrile
B: Water
Gradient  B 10% (0min) → 90% (20min)
Flow rate 0.4 mL/min
Oven 40 ºC
Detection  UV 210 nm
Ghost Trap DS 20 mmL. × 4.0 mmI.D.
Connection to Ghost Trap DS just after the gradient mixer





Two Ghost Trap DS models are now available: the cartridge type for superior cost performance and the 100 MPa high-pressure type for use with UHPLC systems. Select the appropriate one for your application.


Item P/N Description Internal volume *2 Pressure
Ghost Trap DS 228-59921-91 Two cartridges 30 mmL. × 7.6 mmI.D. 35 MPa
228-59921-92 Two cartridges
and one holder
228-59921-93 Two cartridges 20 mmL. × 4.0 mmI.D.
228-59921-94 Two cartridges
and one holder
*1 The life of the product varies according to analysis conditions such as the mobile phase used. The product may deteriorate earlier than the durability test result shown above.
*2 When the product is installed downstream of the gradient mixer or the junction of the pumps, there will be a delay volume equivalent to the internal volume during gradient analysis.
* When a mass spectrometer is used as the detector, the product may cause bleed noise.
* In analysis using an ion-pairing reagent, the ion-pairing reagent may be held by the product, influencing the retention time or peak shape.
* Before connecting an analytical column, be sure to thoroughly clean the flow path attached to the product with the mobile phase (around the final concentration for gradient analysis).
* Please understand that all impurities are not necessarily removed.

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For Research Use Only. Not for use in diagnostic procedures.

This page may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.