Method Scouting

Nexera series

  • Nexera X2 Series
  • Nexera XR
  • Nexera SR
  • Nexera Quarternary
  • Nexera MP
  • Method Scouting

The Nexera Method Scouting System design is based on the Nexera next-generation ultra high performance liquid chromatograph. It uses system uses the special Method Scouting Solution control software and a robust, high-pressure resistant column switching system to switch mobile phases and columns automatically, achieving a quick and accurate method scouting workflow. As a result, this system provides strong support for efficient HPLC method development.

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Maximizing Efficiency for Method Development

 

 

 

 

Enhancing Method Development Efficiency by Nexera Method Scouting

In a typical method scouting procedure, data is collected using a number of mobile phase and column combinations. Analyzers switching manually between these combinations are limited by the number of work hours in a single day, making it impossible to perform method scouting efficiently. The Nexera Method Scouting System is capable of automatically investigating up to 96 combinations of mobile phases and columns, without such time restrictions, thereby significantly improving method development productivity.

 

Method scouting performed by UHPLC, followed by transfer to conventional conditions

In a typical method scouting procedure, data is collected using a number of mobile phase and column combinations. Analyzers switching manually between these combinations are limited by the number of work hours in a single day, making it impossible to perform method scouting efficiently. The Nexera Method Scouting System is capable of automatically investigating up to 96 combinations of mobile phases and columns, without such time restrictions, thereby significantly improving method development productivity.

Method Scouting Workflow

 
With Method Scouting Solution, just set the required conditions to create a scouting batch automatically. This saves time with respect to method creation, management, and batch creation, issues in method development to date. In addition, by combining this system with the Shimadzu’s HPLC workstation, LabSolutions, and utilizing its browser functions, as well as CLASS-Agent and Agent Report, the numerous chromatograms obtained from method scouting can be quickly evaluated, thereby enhancing method development efficiency.

Method Scouting Solution

 
Method scouting results are important data for determining analysis conditions, so obtaining highly reliable data is essential. Method Scouting Solution provides the data required for analysis via a graphical user interface, as well as automated controls conforming to laboratory operations configured in the system settings, thereby providing data reliably.

Achieving automation of laboratory operations

Mobile phase purge conditions and equilibration when automatically switching mobile phases and columns are key issues in method scouting. With Method Scouting Solution, scouting reflects laboratory operations, thanks to automatic control of mobile phase purging and equilibration based on preset system configurations.

 

System confirmation and recording
Data is recorded on system maintenance status prior to scouting, which is
useful for reviews if problems with data occur.

Mobile phase purging
Auto-purge timing is configured. The system performs mobile phase
purging automatically based on the conditions configured here, achieving
fast mobile phase switching.

System equilibration determination
Auto-purge timing is configured. The system performs mobile phase
purging automatically based on the conditions configured here,supporting reliable data acquisition

Scouting scheme
Scouting schemes can be set to give priority to columns or mobile phases
based on laboratory policy.

Integrated settings via column and mobile phase databases

The columns and mobile phases used for method scouting are controlled in a database. During analysis, they are easily selected via a graphical user interface. The names of the selected columns and mobile phases can be reflected in analysis reports. This consolidates notation for the names of the columns and mobile phases used in the laboratory, preventing mistakes with respect to important column and mobile phase conditions when reviewing scouting results.

 

Graphical data provided to support reliable analysis

 

Method scouting uses a variety of mobile phase and column combinations. Method Scouting Solution calculates the required amounts of these mobile phases and samples in advance, and provides this data to avoid shortages. Furthermore, upper pressure limits are automatically controlled based on column pressure resistance data registered in the column database, thereby avoiding degradation of precious columns. In addition, the system provides estimates of completion time, with consideration to the time required between analyses for processes such as mobile phase auto-purging and column equilibration. As a result, scouting can be configured to suit your work schedule.

Fast data browsing

 

The ability to quickly search the large quantity of data obtained from method scouting for suitable conditions is important. The data browser function provided with LabSolutions meets this need by displaying a list of these chromatograms and analysis results. In addition to retention time, area, and height, analysis results include the degree of separation, separation coefficients, theoretical plate numbers, symmetry coefficients and other indices for chromatogram evaluation, supporting the process of checking the large amount of data for optimal conditions.

Quantitation of scouting results via CLASS-Agent Report

CLASS-Agent Report enables quantitation in order to search the data obtained from method scouting for optimal data. With CLASS-Agent Report, numerical data and graphs can be created, utilizing Microsoft Excel, from calculations based on the degree of separation and the number of peaks. As a result, in addition to visual comparison of chromatograms, determinations can be made based on quantitation of determination standards.

 

 

Providing Highly Reliable Data

The Nexera Method Scouting System is based on the Nexera UHPLC,which has an its established reputation for ultra-high speed analysis.As a result, highly reliable method scouting results are provided using ultra-high speed analysis featuring stable solvent delivery at ultra-high pressures, excellent injection repeatability from low injection quantities, and ultra-low carryover.

Basic configuration of the Nexera Method Scouting System

The Nexera Method Scouting System achieves stable data acquisition by adopting the newly developed FCV-34AH (100 MPa pressure resistance) high-pressure-resistant column switching valve and manifold. In addition, with auto-purging, mobile phase equilibration times are reduced by purging the mobile phase inside the autosampler as well, which means a faster method scouting workflow.

Basic configuration of the Nexera Method Scouting System

Enabling graphical display of column connections

The piping for 6 columns is color coded and interlinked with the graphical user interface, an approach that enables reliable settings. This makes it possible to reduce the risk of column setting mistakes during analysis.

Column connections interlinked with the graphical user interface.

 

 

 

 

 

 

 

 

NEWS / EVENT

March 26, 2018
January 16, 2018

For Research Use Only. Not for use in diagnostic procedures.

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