HPLC Columns

Shim-pack GIST/GISS/GIS/GWS Series

  Shim-pack GIST Series Shim-pack GISS Series Shim-pack GIS Series Shim-pack GWS Series
Features
Ultra-high inertness and high durability
High-speed analysis with ultra-high

inertness and high durability
High retentivity, lower column back pressure,

high inertness
Medium retentivity and high inertness
Product
Lineup
C18, C8, Phenyl, NH2 C18 C18, CN C18
Particle Size
2, 3, 5 (NH2: 3, 5)
1.9, 3, 5
2, 3, 4, 5, 10 (CN: 3, 5)
5
pH Range
1-10

(Phenyl, NH2: 2-7.5)
1-10 2-7.5 2-7.5

Shim-pack GIST Series

Ultra-high inertness and high durability

Shim-pack GIST series columns are packed with newly developed high-purity porous spherical silica. Superior inertness not only improves analytical precision, but also increases column durability. Shim-pack GIST series is capable of tolerating a wider pH (1-10) situation, making it suitable for analysis of many different compounds.

C18, C8, Phenyl and NH2 columns are offered to cover an extended range of selectivity and meet diverse research and development needs.

Furthermore, Shim-pack GIST series is designed with various particle size ranges, which make it easy to transfer your analysis from ultra-high-speed to conventional methods.

Analysis Examples

Nine Pesticides on a Golf Course

■ Conditions
Column  Shim-pack GIST-HP C18

 (150 mmL. x 3.0 mmI.D., 3 um)
Eluent  A) 50 mM KH2PO4 (pH 3.5, H3PO4)

 B) CH3CN

 A/B = 60/40 -4 min- 40/60
Flow Rate  1.5 mL/min
Col. Temp.  40 °C
Detection  UV 235 nm
Injection Vol.  5.0 μL

Sugars

■ Conditions
Column  Shim-pack GIST NH2

 (250 mmL. x 4.6 mmI.D., 5 um)
Eluent  A) H2O

 B) CH3CN

 A/B = 25/75, v/v
Flow Rate  1.0 mL/min
Col. Temp.  40 °C
Detection  RID
Injection Vol.  5 μL
 

Specifications

  Shim-pack

GIST C18
Shim-pack

GIST C8
Shim-pack

GIST Phenyl
Shim-pack

GIST NH2
Bonded Phase
Octadecyl
Groups
Octyl Group Phenyl Group
Aminopropyl
Group
Analysis Mode
Reversed
Phase
Reversed
Phase
Reversed
Phase
HILIC mode,
Normal Phase
Features
Ultra-high inertness and high durability
Ultra-high inertness and high durability
Sugar analysis
Particle Size (μm) 2, 3, 5 2, 3, 5 2, 3, 5 3, 5
Pore Size (nm) 10 10 10 10
Surface Area (m2/g)
350 350 350 350
Carbon Loading (%)
14 8 10 7
End Cap - -
Inertness ▶▶▶▶▶ ▶▶▶▶▶ ▶▶▶▶▷ -
pH Range 1-10 1-10 2-7.5 2-7.5
 

Shim-pack GISS Series

 
Shim-pack GISS series maintains the same ultra-high inertness and wider pH range as the Shim-pack GIST series while providing rapid separations with symmetric peaks. The optimization of surface area, pore size and chemical bonding delivers superior peak shapes, making these columns ideal for LC/MS/MS analysis.

Shim-pack GISS series also includes the 1.9μm and 3μm HP series for UHPLC analyses.

Analysis Examples

β-blocker

■ Peaks (100 μg/L each)
   Q1 > Q3
 1. Acebutolol  337.10 > 116.05 (+)
 2. Atenolol  267.25 > 145.00 (+)
 3. Labetalol  329.00 > 161.95 (+)
 4. Nadolol  310.05 > 254.00 (+)
 5. Pindolol  249.80 > 116.00 (+)
 
■ Conditions
Column  Shim-pack GISS C18 (50 mmL x 2.1 mmI.D., 1.9 um)
Eluent  A) 10 mM Ammonium Formate in H2O

 B) 10 mM Ammonium Formate in CH3OH

 A/B = 70/30 ‒ 0.3min - 40/60 ‒ 0.5min - 0/100 ‒ 0.1min ‒ 0/100 ‒ 0.01min -70/30 ‒ 0.5min - 70/30 ,v/v
Flow Rate  0.6 mL/min
Col. Temp.  40 °C
Detection  LC/MS/MS(ESI, Positive, Negative MRM)

Specifications

  Shim-pack GISS C18
Bonded Phase Octadecyl Groups
Analysis Mode Reversed Phase
Features
High-speed analysis with ultra-high inertness and high durability
Particle Size (μm) 1.9, 3, 5
Pore Size (nm) 20
Surface Area (m2/g)
200
Carbon Loading (%)
9
End Cap
Inertness ▶▶▶▶▶
pH Range 1-10
 

Shim-pack GIS Series

High retentivity, lower column back pressure, high inertness

Shim-pack GIS series columns are packed with high purity silica gel and are your ideal generic HPLC columns.

Highly uniform particles ensure stable mobile phase delivery and outstanding low pressure. The Shim-pack GIS series not only reduces your cost of solvents, but relieves your system load as well. A uniform silica surface and stable chemical modification ensure high analysis reproducibility.

Analysis Examples

Food Dyes

■ Peaks
 1. Tartrazine  7.6 mg/L
 2. Amaranth  3.8 mg/L
 3. Ingigocarmine  7.6 mg/L
 4. New coccine  3.8 mg/L
 5. Sunset Yellow FCF  5.3 mg/L
 6. Naphthol Yellow S  7.6 mg/L
 7. Uranine  3.8 mg/L
 8. Allura Red AC  5.3 mg/L
 9. Ponceau R  7.6 mg/L
 10. Ponceau SX  5.3 mg/L
 11. Orange I  5.3 mg/L
 12. Fast green FCF  3.0 mg/L
 13. Brilliant blue FCF  3.0 mg/L
 14. Ponceau 3R  7.6 mg/L
 15. Erythrosine  5.3 mg/L
 16. Azure Blue VX  3.0 mg/L
 17. Orange II  7.6 mg/L
 18. Acid red  3.0 mg/L
 
■ Conditions
Column  Shim-pack GIS C18 (150 mmL. x 4.6 mmI.D, 4 um)
Eluent  A) 10mM Na2HPO4 (pH6.9 ; 10mM NaH2PO4)

 B) CH3CN

 A/B = 90/10 - 50 min - 65/35
Flow Rate  1.0 mL/min
Detector  UV 270nm
Col. Temp.  40 °C
Injection Vol.  10 μL

Free Amino acids and Organic acids

■ Conditions
 Column  Shim-pack GIS CN

 (250 mmL. x 4.6 mmI.D., 5 um)
 Eluent  20 mM KH2PO4 (pH 4.0; H3PO4)
 Flow Rate  1.0 mL/min
 Col. Temp.  40 °C
 Detection  UV 210 nm
 Injection Vol.  5 μL
 

Specifications

  Shim-pack GIS C18 Shim-pack GIS CN
Bonded Phase Octadecyl Groups Cyanopropryl Group
Analysis Mode Reversed Phase Reversed Phase, Normal Phase
Features
High retentivity, lower column
back pressure, high inertness
Suitable in either reversed
phase or normal phase mode
Particle Size (μm) 2, 3, 4, 5, 10 3, 5
Pore Size (nm) 10 10
Surface Area (m2/g)
450 450
Carbon Loading (%)
15 14
End Cap -
Inertness ▶▶▶▶▷ -
pH Range 2-7.5 2-7.5
 

Shim-pack GWS Series

Medium retentivity and high inertness

Shim-pack GWS series is an economical generic C18 column designed with high cost performance.

A uniform pore size ensures low pressure. Complete end-capping makes it possible to be used for analysis of acidic or basic compounds. Shim-pack GWS series is the choice for cost control.

Analysis Examples

Cold medication

■ Conditions
 Column  Shim-pack GWS C18

 (150 mmL. x 4.6 mmI.D., 5 um)
 Eluent  A) 0.1%H3PO4

 B) CH3CN

 A/B = 90/10 -2min- 90/10 -10min- 0/100
 Flow Rate  1.0 mL/min.
 Col. Temp.  40 °C
 Detection  UV 210 nm
 Injection Vol.  10 μL
 

Specifications

  Shim-pack GWS C18
Bonded Phase Octadecyl Groups
Analysis Mode Reversed Phase
Features
Economical C18 column with medium retentivity and high inertness
Particle Size (μm) 5
Pore Size (nm) 10
Surface Area (m2/g)
450
Carbon Loading (%)
9.5
End Cap
Inertness ▶▶▶▶▷
pH Range 2-7.5
 

Catalog

Shim-pack GIST/GISS/GIS/GWS Series

 

Reversed Phase and Normal Phase HPLC Columns

Shim-pack

GIST/GISS/GIS/GWS Series


(pdf 2,683kB)
 



 

* Appearance and specifications are subject to change without notice.

 

For Research Use Only. Not for use in diagnostic procedures.



This page may contain references to products that are not available in your country.

Please contact us to check the availability of these products in your country.

 

For Research Use Only. Not for use in diagnostic procedures.

This page may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.