Features

Automated Pretreatment Module for Cell Culture Media Analysis

Up to 65 samples can be pretreated automatically.

The probe for the culture supernatant sample ((3) sample probe) and the probe for the internal standard and organic solvent used as the deproteinization agent ((4) reagent probe) are separated, limiting cross contamination between samples.

 

Everything from pretreatment to analysis can be carried out with the common sample ID.

 

In the control software, the progress of pretreatment and analysis is easily confirmed.

Using the dedicated software, analysis operations are performed intuitively. No complicated method settings are required.

 

 

The Optimal Workflow Can Be Selected

The C2MAP system supports two modes: Batch mode (ordinarily used) that gives priority to the pretreatment of the culture supernatant samples placed, and sequential mode in which pretreatment and analysis are performed alternately for each sample.

 

Performing only the sample pretreatment process is possible in normal mode.

 

Supports a Wide Range of Measurement Compounds and Culture Supernatant Samples

The analysis method stored in the control software allows the simultaneous analysis of the following 95 components plus 2-Isopropylmalic acid.

 

It is confirmed that the C2MAP system can accommodate the follow ing culture media and culture media additives.

Cell Type CHO cells iPS/ES cells T cells Mesenchymal Cell Type
Culture Media BalanCD® CHO AK03N X-VIVO™ 10 MSCBM
1×CD CHO Essential-8™ X-VIVO™ 15 MesenPRO™
EX-CELL® CHO mTeSR™1/TeSR™-E8™ TexMACS™ Stempro®
Additives Fetal Bovine Serum(100% v/v)

 

C2MAP TRENDS

For results obtained via LC/MS/MS, temporal changes in each component can be graphed by the dedicated viewer software. Analysts can monitor variations in metabolites secreted from cells and culture media components during cultivation, as well as display graphs of component comparisons with samples from different culture series. As a result, the consumption and depletion of culture media components, and changes in the amounts of metabolic components secreted from cells, can be observed, thereby providing useful insights into considerations of the optimal culture conditions and assessments of cellular status.

◆Temporal Changes in Measured Components

◆Measured-component Comparisons among Different Culture Series

 

NEWS / EVENT

For Research Use Only. Not for use in diagnostic procedures.

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