addition to the hydrophobic characteristics of the ODS, the metal content also provides cation-exchange effects. This increases the retention of basic compounds. Therefore, this allows use with only a buffer solution as the mobile phase for analyses that previously required using an ion pair reagent and enables using gradient elution.
| Column size | 150 mmL. x 4.6 mmI.D., 5 μm |
|---|---|
| Mobile phase |
A) 10 mmol/L phosphate (Na) buffer
solution (pH 2.6) B) Acetonitrile
|
| Time program | B Conc. 1% (0 min) → 1% (2.5 min) → 50% (10 min) → 1% (10.01 min → 15 min) |
| Detection | UV 210 nm |
| Flow rate | 1.2 mL/min |
| Injection volume |
10 μL |
| Column temperature | 40 °C |
| Column size | 150 mmL. x 4.6 mmI.D., 5 μm |
|---|---|
| Mobile phase |
A) 100 mmol/L phosphate (Na) buffer
solution (pH 2.1) containing 0.8 mmol/L sodium 1-octanesulfonate B) Acetonitrile A/B = 10/1 (v/v)
|
| Detection | UV 210 nm |
| Flow rate | 1.2 mL/min |
| Injection volume |
10 μL |
| Column temperature | 40 °C |
| Column size | 150 mmL. x 4.6 mmI.D., 5 μm |
|---|---|
| Mobile phase |
A) 10 mmol/L phosphate (Na) buffer
solution (pH 2.6) B) Acetonitrile
|
| Time program | B Conc. 8% (0 min) → 8% (5 min) → 50% (12 min) → 8% (12-20 min) |
| Detection | UV 254 nm |
| Flow rate | 1.0 mL/min |
| Injection volume |
5 μL |
| Column temperature | 25 °C |
| Column size | 150 mmL. x 4.6 mmI.D., 5 μm |
|---|---|
| Mobile phase |
2 g of sodium 1-heptanesulfonate
was dissolved in 900 mL of water and acetic acid (100) was added to produce a pH of 3.0. Then water was added to make 1000 mL. 240 mL of acetonitrile and 40 mL of methanol were added to this solution. |
| Detection | UV 254 nm |
| Flow rate | 0.5 mL/min |
| Injection volume |
5 μL |
| Column temperature | 25 °C |
|
Mobile phase
|
A) 20 mmol/L phosphate (Na) buffer solution (pH 2.5)
B) Acetonitrile
|
|---|---|
|
Time program
|
B Conc.1% (0 min) → 1% (2min) → 50% (8 min) → 50% (18 min) → 1% (18.01 min → 23 min)
|
|
Detection
|
UV 220 nm
|
|
Flow rate
|
1.0 mL/min
|
|
Injection volume
|
10 μL
|
|
Column
temperature |
40 °C
|
| Product Name | Internal Diameter |
Length
|
Particle Size | Max. Operating Pressure |
|
Shim-pack MAqC-ODS I
|
4.6 mm | 150 mm | 5 μm | Approx. 20 MPa |
* Appearance and specifications are subject to change without notice.
For Research Use Only. Not for use in diagnostic procedures.
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Please contact us to check the availability of these products in your country.
To use this column efficiently: 1) To increase the retention of basic compounds, please use a buffer solution within the pH 2 to 4 range. 2) In the case of a basic substance tailing, it may be possible to improve the peak shape by increasing the salt concentration of a buffer solution. 3) The elution of basic compounds is faster by increasing the salt concentration, and it is possible to adjust retention by salt concentration.
For Research Use Only. Not for use in diagnostic procedures.
This page may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.